DNA

Part:BBa_K676007

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


Gyrase A

This is the wild type Gyr A ORF (2627bp) from E.coli , the GyrA subunit is responsible for catalysing the breakage and resealing of DNA strands . A full functional gyrase consist of two GyrA and two GyrB . Gyr A has important tyr122 residue which is involved in forming the phosphotyrosine linkage with the 5' end of the DNA strand in the active site. The full structure of the gyrase is modelled and can be found in our wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1593
    Illegal BglII site found at 2538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 27
    Illegal AgeI site found at 703
    Illegal AgeI site found at 760
    Illegal AgeI site found at 1399
    Illegal AgeI site found at 2155
    Illegal AgeI site found at 2523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43
    Illegal SapI.rc site found at 51
    Illegal SapI.rc site found at 1510


[edit]
Categories
Parameters
None